Journal: Magnetic Resonance in Chemistry

Article Title: 13 C SPE MAS measurement of ligand concentration in compressible chromatographic beads

doi: 10.1002/mrc.4257

Figure Lengend Snippet: The ligand and linker to the agarose resin in the Butyl Sepharose High Performance chromatography medium.

Article Snippet: In this article, we develop a protocol, based on a 13 C single pulse excitation (SPE) magic angle spinning (MAS) NMR experiment, for measuring the concentration of butyl ligands in the HIC medium Butyl Sepharose™ High Performance (GE Healthcare Bio‐Sciences AB, Uppsala, Sweden).

Techniques: Chromatography

Journal: Magnetic Resonance in Chemistry

Article Title: 13 C SPE MAS measurement of ligand concentration in compressible chromatographic beads

doi: 10.1002/mrc.4257

Figure Lengend Snippet: 13 C spectra for the medium hydrated at 95% relative humidity with the internal reference DMS. From bottom to top: background spectrum with empty rotor (red), spectrum of medium and DMS (blue), and spectrum of medium and DMS after background subtraction and baseline correction (black). The broad peak in the 60–120 ppm range arises from the agarose resin and also contains the butyl ligand C1 (carbon closest to the ether linker) peak. Left inset: magnification showing the spectral region with the DMS and butyl peaks in the background and baseline‐corrected spectrum. The DMS (42.5 ppm), butyl C2 (32 ppm), C3 (19 ppm) and C4 (14 ppm) peaks were integrated in the ranges shown. Right inset: spectrum for resin before ligand attachment. The small peaks between 14–23 ppm are due to moieties on the resin that contribute to the C3/C4 integrals in the ligand‐coupled resin.

Article Snippet: In this article, we develop a protocol, based on a 13 C single pulse excitation (SPE) magic angle spinning (MAS) NMR experiment, for measuring the concentration of butyl ligands in the HIC medium Butyl Sepharose™ High Performance (GE Healthcare Bio‐Sciences AB, Uppsala, Sweden).

Techniques: